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Journal: medRxiv
Article Title: Molecular epidemiology of rifampicin resistant Mycobacterium tuberculosis in Vietnam
doi: 10.64898/2026.04.20.26351312
Figure Lengend Snippet: Geography of main M. tuberculosis lineages in Vietnam. A) Maximum-liklihood tree of 287 M. tuberculosis isolates from the VQUIN MDR trial. Nodes in bold indicate lineage 2.2.1. Red stars used to indicate isolates from household contacts B) Proportion of lineages represented in each region sampled. Quang Nam was excluded as it only had a single representative isolate (sublineage 2.2.1).
Article Snippet: New genome sequences were phylogenetically contextualised to publicly-available
Techniques:
Journal: medRxiv
Article Title: Direct detection and quantification of Mycobacterium tuberculosis from clinical samples by high-resolution melt qPCR
doi: 10.64898/2026.03.07.26347851
Figure Lengend Snippet: (A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
Article Snippet: Using the
Techniques: Amplification, Control, Generated