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ATCC virus strains m tuberculosis h37rv lab stock atcc 27294 m tuberculosis h37ra lab stock atcc 25177 cell lines hepg2 cells
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Biotechnology Information m tuberculosis genome sequences
Geography of main M. <t>tuberculosis</t> lineages in Vietnam. A) Maximum-liklihood tree of 287 M. tuberculosis isolates from the VQUIN MDR trial. Nodes in bold indicate lineage 2.2.1. Red stars used to indicate isolates from household contacts B) Proportion of lineages represented in each region sampled. Quang Nam was excluded as it only had a single representative isolate (sublineage 2.2.1).
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ATCC m tuberculosis h37rv
(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
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ATCC reference genome m tuberculosis h37rv atcc 27294
(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
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(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
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ATCC m tuberculosis strain h37rv
(A) Amplification plot of <t>H37Rv</t> replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.
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Geography of main M. tuberculosis lineages in Vietnam. A) Maximum-liklihood tree of 287 M. tuberculosis isolates from the VQUIN MDR trial. Nodes in bold indicate lineage 2.2.1. Red stars used to indicate isolates from household contacts B) Proportion of lineages represented in each region sampled. Quang Nam was excluded as it only had a single representative isolate (sublineage 2.2.1).

Journal: medRxiv

Article Title: Molecular epidemiology of rifampicin resistant Mycobacterium tuberculosis in Vietnam

doi: 10.64898/2026.04.20.26351312

Figure Lengend Snippet: Geography of main M. tuberculosis lineages in Vietnam. A) Maximum-liklihood tree of 287 M. tuberculosis isolates from the VQUIN MDR trial. Nodes in bold indicate lineage 2.2.1. Red stars used to indicate isolates from household contacts B) Proportion of lineages represented in each region sampled. Quang Nam was excluded as it only had a single representative isolate (sublineage 2.2.1).

Article Snippet: New genome sequences were phylogenetically contextualised to publicly-available M. tuberculosis genome sequences sampled in Vietnam from National Center for Biotechnology Information (NCBI) Sequence Read Archives (SRA).

Techniques:

(A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Journal: medRxiv

Article Title: Direct detection and quantification of Mycobacterium tuberculosis from clinical samples by high-resolution melt qPCR

doi: 10.64898/2026.03.07.26347851

Figure Lengend Snippet: (A) Amplification plot of H37Rv replicates targeting the RD9 gene. (B) Corresponding melt curve showing a specific melting temperature (Tm) of ∼75°C. H37Rv DNA was serially diluted from 10 6 to 10 1 copies per reaction and analysed in triplicate. (C) Amplification plot obtained after 40 cycles for the dilutions, including the no-template control (NTC). (D) Corresponding melt curve showing a Tm of 73.7 ± 0.12°C. (E) Standard curve generated from the 10-fold serial dilutions, plotting cycle threshold (Ct) versus log copy number per reaction.

Article Snippet: Using the M. tuberculosis H37Rv (ATCC #25618DQ) reference strain, we performed asymmetric PCR with Vent (exo-) DNA polymerase (New England Biolabs).

Techniques: Amplification, Control, Generated